Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study

Abstract Background In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level expression. Therefore, rational fine-tuning to regulate levels target genes for optimizing biosynthetic pathways in metabolic engineering has recently become an active area research. Results this study, we systematically detected characterized common promoter elements unconventional yeast Yarrowia lipolytica , constructed artificial hybrid library that covers a wide range strength. The results indicate strength can be fine-tuned elements, namely, upstream activation sequences (UAS), TATA box core promoter. Notably, UASs Saccharomyces cerevisiae were reported first time functionally transferred Y. . Subsequently, using production versatile platform chemical isoamyl alcohol as test was applied optimize biosynthesis pathway By expressing key gene, ScARO10 with library, 1.1–30.3 folds increase titer over control strain Po1g KU70 ∆ achieved. Interestingly, highest attained weak P UAS1B4-EXPm express These suggest our powerful toolkit identifying optimum Conclusion We envision strategy rationally engineered study could also extended other non-model fungi improvement.